HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ivanova, P. T. Articles by Brown, H. A. Search for Related Content PubMed PubMed Citation Articles by Ivanova, P. T. Articles by Brown, H. A.

LIPID Arrays: New Tools in the Understanding of Membrane Dynamics and Lipid Signaling

Department of Pharmacology and the Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232

Correspondence: Address correspondence to HAB. E-mail alex.brown{at}vanderbilt.edu; fax 615-343-6532.

Phospholipids are the structural building blocks of the membrane bilayer, which retains and regulates intra-cellular content. In addition to creating a protective barrier around the cell, lipids modulate membrane trafficking and are themselves precursors of important intracellular signaling molecules. Identification and quantification of these molecular species is essential for a more complete understanding of cell signaling pathways, and more reliable and sensitive methods are needed for determining membrane phospholipid content. Recent improvements in electrospray ionization mass spectrometry have made possible the direct identification of more than 400 phospholipid species from biological extracts of a single cell type. Changes in the cellular concentration of diverse lipids can be determined by analysis of the mass spectra by statistical algorithms. In the future, lipid arrays will be integrated with other high-throughput profiling technologies, and computational lipidomics will expand our understanding of the molecular basis of cellular processes and diseases.

This article has been cited by other articles:

				W. Hou, H. Zhou, F. Elisma, S. A. L. Bennett, and D. Figeys Technological developments in lipidomics Brief Funct Genomic Proteomic, September 19, 2008; (2008) eln042v1. [Abstract] [Full Text] [PDF]

				G. R. Kinsey, J. L. Blum, M. D. Covington, B. S. Cummings, J. McHowat, and R. G. Schnellmann Decreased iPLA2{gamma} expression induces lipid peroxidation and cell death and sensitizes cells to oxidant-induced apoptosis J. Lipid Res., July 1, 2008; 49(7): 1477 - 1487. [Abstract] [Full Text] [PDF]

				J. Gomez-Cambronero, M. Di Fulvio, and K. Knapek Understanding phospholipase D (PLD) using leukocytes: PLD involvement in cell adhesion and chemotaxis J. Leukoc. Biol., August 1, 2007; 82(2): 272 - 281. [Abstract] [Full Text] [PDF]

				A. D. Watson Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders. Lipidomics: a global approach to lipid analysis in biological systems J. Lipid Res., October 1, 2006; 47(10): 2101 - 2111. [Abstract] [Full Text] [PDF]

				E. Fahy, S. Subramaniam, H. A. Brown, C. K. Glass, A. H. Merrill Jr., R. C. Murphy, C. R. H. Raetz, D. W. Russell, Y. Seyama, W. Shaw, et al. A comprehensive classification system for lipids J. Lipid Res., May 1, 2005; 46(5): 839 - 862. [Abstract] [Full Text] [PDF]